LAG3:FGL1[Biotinylated] Inhibitor Screening Assay Kit

LAG3 (lymphocyte-activation gene 3), also known as CD223, is a cell surface receptor that functions as an inhibitory immune checkpoint. It is found in CD4+and CD8+T cells, NK (natural killer) cells, NKT cells and Treg (T regulatory) cells.  LAG3 is involved in T cell exhaustion and proliferation. It has several ligands, which include galectin-3, α-synuclein and Fibrinogen-like protein 1 (FGL1) . LAG3 and FGL1 are found in tumor cells, where they can serve as immune checkpoints and potentially as oncogenes, and their levels correlate with patient prognosis.  LAG3/FGL1 plays a role in immune cell function, cytokine production and tumor growth and metastasis. Several monoclonal antibodies targeting LAG3 are currently being tested, such as relatlimab, and have shown promising results alone or in combination therapy. Blockade of the FGL1-LAG3 interaction can promote antitumor immunity and may benefit patients that have developed tumor immune resistance. For instance, high levels of FGL1 may contribute to gefitinib therapy resistance while high LAG3 levels were found in patients after EGFR (epidermal growth factor receptor) -TKI (tyrosine kinase inhibitor) treatment. A deeper understanding of the importance of this complex in health and disease will allow the development of new cancer therapy strategies.

Catalog #NameAmountStorage71229LAG3, His-Tag*50 µg-80°C100327FGL1, Fc-Fusion (IgG1), Avi-Tag, Biotin-Labeled5 µg-80°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79742Streptavidin-HRP10 µl+4°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom Temp7969996-well white nickel coated microplate1+4°C*The concentration of the protein is lot-specific and will be indicated on the tube.

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