VSIG-3:VISTA [Biotinylated] Inhibitor Screening Assay Kit

The VSIG-3:VISTA [Biotinylated] Inhibitor Screening Assay Kit is a chemiluminescent ELISA designed to measure the binding between VSIG-3 (V-set and Ig domain-containing protein 3) and VISTA (V-domain immunoglobulin suppressor of T-cell activation, also known as B7-H5) protein for screening and profiling applications. This kit comes with enough purified VSIG-3 (amino acids 22-244) and biotinylated VISTA (amino acids 33-194), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. VSIG-3:VISTA [Biotinylated] Inhibitor ScreeningAssay Kit schematic.A 96-well plate is coated with VSIG-3protein. After coating and blocking, biotinylated VISTAis added in an optimized assay buffer. Next, unbound biotinylated VISTA is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of VISTA binding to VSIG-3.Need us to run inhibitor screens or profile your compounds against VSIG-3:VISTA [Biotinylated]? Check out ourImmunotherapy Biochemical Screening Services.