PI3Kα (p110α/p85) Assay Kit

PI3Ks (Phosphatidylinositol 3-kinases) are lipid kinases that phosphorylate PIP2 (phosphatidylinositol 4,5-bisphosphate) to produce PIP3 (phosphatidylinositol 3,4,5-trisphosphate), which plays important roles in fundamental cellular activities such as cell growth, survival, migration, and metabolism. In human cancers, gain-of-function mutations of PI3Ks are found frequently suggesting that PI3Ks are closely involved in tumorigenesis and PI3K targeting inhibitors may be promising anticancer drug candidates.

CatalogNumberReagentAmountStorage40620PI3Kα (p110α/p85α) 2 µg-80°CAvoid multiplefreeze/thawcycles!793345x Kinase assay buffer1.5 ml-20°C79686ATP (500 μM) 100 µl-20°C40560PI3K lipid substrate (Packaged separately, DO NOT FREEZE) 500 µl4°C96-well plate, white1Room Temp.