HPK1 Assay Kit

The HPK1 Assay Kit is designed to measure HPK1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in-vitro kinase activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.

Product Specifications

Background

HPK1 (MAP4K1) or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family. It is a tissue-specific upstream activator of the MEKK/JNK/SAPK signaling pathway. HPK1 diminishes T cell receptor (TCR) signaling activity and T cell proliferation by phosphorylating the adaptor protein SLP-76, suggesting HPK1 could be a novel target for anti-tumor immunotherapy.

UniProt

Q92918

Format

Catalog #ComponentsAmountStorage40398HPK13 µg-80°CAvoid freeze/ thaw cycles!793345x Kinase assay buffer1.5 ml-20°C79686ATP (500 µM) 100 µl-20°CMBP (5 mg/ml) 200 µl-20°C7969696-well plate, white1Room Temp.