TNFR2:TNF-alpha[Biotinylated] Inhibitor Screening Assay Kit

The TNFR2: TNFalpha[Biotinylated] Inhibitor Screening Assay Kit is designed to measure the binding of TNFR2 (tumor necrosis factor receptor 2) to TNF-alpha (TNFα) for screening and profiling applications. The TNFR2:TNF-alpha [Biotinylated] Inhibitor Screening Assay Kit comes in a convenient 96-well format, with enough recombinant purified biotinylated TNF-alpha (amino acids 29-216), TNFR2 (amino acids 23-257), blocking and assay buffer and detection reagents for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against TNFR2:TNF-alpha[Biotinylated]? Check out ourImmunotherapy Biochemical Screening Services.This product has beencited 6 times.

Product Specifications

Background

Tumor Necrosis Factor Receptor 2 (TNFR2, also known as TNFRSF1B or CD120b) is a transmembrane receptor of the TNF protein superfamily that binds the pleiotropic pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) . TNFR2 can be found in several T cell subsets such as regulatory CD8+T cells (Tregs) and CD4+tumor-infiltrating T cells, myeloid lineage cells and some cancer types, and it is involved in autoimmune diseases, graft versus host disease and cancer. It has become an attractive target for cancer immunotherapy, where its different functions as oncogene and immune regulator are being explored. TNFR2 also exhibits neuroprotective properties and promotes tissue regeneration, making it a promising potential therapeutic target for the treatment of Alzheimer’s disease.

UniProt

TNFR2: P20333; TNF-alpha: P01375

Applications

Screening of small molecules and antibodies that inhibit binding of TNFR2 to TNF-alpha.

Format

Catalog #NameAmountStorage79363TNFR2, Fc-Fusion (IgG1), His-Avi-Tag*10 µg-80°CTNF-alpha, Biotin-Labeled*2 µg-80°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79742Streptavidin-HRP10 µl+4°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom Temp7969996-well white microplate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]