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LIGHT:HVEM [Biotinylated] Inhibitor Screening Assay Kit

The LIGHT:HVEM[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of LIGHT:HVEM signaling. The key to this kit is the high sensitivity of detection of biotin-labeled HVEM by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LIGHT is coated on a 96-well plate. Next, HVEM is incubated with LIGHT on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against LIGHT:HVEM [Biotinylated]? Check out ourImmunotherapy Biochemical Screening Services.

Product Specifications

Background

Herpesvirus entry mediator (HVEM, also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14) or CD270) and LIGHT (tumor necrosis factor superfamily member 14 (TNFSF14) or CD258) mediate costimulatory responses in T cell activation and proliferation during the immune response. The HVEM/LIGHT co-signaling pathway plays important roles in several biological processes, including autoimmunity, inflammation, bacterial and virus infection, transplant rejection and cancer.

UniProt

LIGHT: O43557; HVEM: Q92956

Format

CatalogNumberComponentAmountStorage71266LIGHT, His-Tag (Human) 10 µg-80 °CAvoidmultiplefreeze/thawcycles!71143HVEM, Fc fusion, Biotin-labeled (Human) 5 µg-80 °C793113x Immuno Buffer 150 ml-20 °C79728Blocking Buffer 250 ml+4 °CStreptavidin-HRP15 µl-20 °C79670ELISA ECL substrate A (transparent bottle) 6 mlRoomTempELISA ECL substrate B (brown bottle) 6 mlRoomTemp7969996-well white microplate1+4 °C

Shipping Conditions

-80°C

Storage Conditions

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Frequently Asked Questions

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