BAFF:BCMA[Biotinylated] Inhibitor Screening Assay Kit

The BAFF:BCMA[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of BAFF:BCMA signaling.The key to this kit is the high sensitivity of detection of biotin-labeled BCMA by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, BAFF is coated on a 96-well plate. Next, BCMA is incubated with BAFF on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against BAFF:BCMA[Biotinylated]? Check out ourImmunotherapy Biochemical Screening Services.

Product Specifications

Background

The TNF family ligand B-cell Activating Factor (BAFF, also known as BLyS, TALL-1 or CD257, encoded by the TNFSF13B gene) is a Type II membrane-bound protein, which can be released as a soluble ligand upon proteolytic processing. This cytokine is a ligand for the receptors Transmembrane Activator and CAML Interactor (TACI, TNFRSF13B), BAFF Receptor (BAFF-R, TNFRSF13C) and B-cell Maturation Antigen (BCMA, TNFRSF17) . These interactions promote cell survival and they play a crucial role in B cell development. In particular, BCMA signaling is an important player in the later stages of B cell differentiation, including the survival of long-lived bone marrow plasma cells and likely for the survival of plasmablasts.

UniProt

BAFF: Q9Y275; BCMA: Q02223

Format

Catalog #ComponentAmountStorage100194BAFF, His-Avi-tag HiP™10 µg-80°CAvoidfreeze/thawcycles79467BCMA, Fc-fusion (IgG1), Avi-Tag, Biotin-Labeled HiP™5 µg-80°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer50 ml+4°C79742Streptavidin-HRP10 µl-20°C79670ELISA ECL substrate A (transparent bottle) 6 mlRoom TempELISA ECL substrate B (brown bottle) 6 mlRoom Temp7969996-well white microplate1+4°C