ALK2 (ACVR1) Kinase Assay Kit

The ALK2 (ACVR1) Kinase Assay Kit is designed to measure ALK2 (activin receptor-like kinase-2) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant ALK2 (amino acids 147-509), casein, ATP, and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against ALK2? Check out ourKinase Screening Services.

Product Specifications

Background

ALK2 (activin receptor-like kinase-2), also known as ACVR1 (activin A receptor, type 1) is a protein of the BMP (bone morphogenetic protein) and TGFβ (transforming growth factor beta) family, and is a type I receptor. Activin signaling occurs by binding activin to heteromeric complexes of receptor type I and type II, with receptor type I being responsible for signaling and type II for ligand binding. SMAD1 (mothers against decapentaplegic homolog 1) /2/3/6 are activated by ALK2. This protein is involved in the development of several organs, such as heart, nervous system, and bone. Dysfunction of ALK2 can result in a set of rare diseases, namely fibrodysplasia ossificans progressive (FOP) . ALK2 is also linked to DIPG (diffuse intrinsic pontine glioma) . The development of ALK2 inhibitors has been hindered by the close similarity in the kinase domain of the 7 known ALK, with particular attention needed on inhibition of ALK5, which can result in cardiac toxicity. Clinical trials are ongoing and will pave the way for further studies using ALK2-specific inhibitors.

UniProt

Q04771

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage40019ALK2 (ACVR1), GST-Tag10 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP100 µl-20°C79550Casein (5 mg/ml) 100 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]