BCL2A1 TR-FRET Assay Kit

The BCL2A1 TR-FRET Assay Kit is designed to measure the inhibition of BCL2A1 (BFL1) binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL2A1, peptide ligand, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.Need us to run inhibitor screens or profile your compounds against BCL2A1? Check out ourApoptosis Screening Services.

Product Specifications

UniProt

Q16548

Format

Catalog NumberComponentAmountStorage100077BCL2A120 µg80°CAvoidfreeze/thawcycles!BCL2 Peptide Ligand 2400 rxns80°C30017Anti-His Tb-labeled donor2 x 10 µl-20°CDye-labeled acceptor2 x 10 µl-20°C3x MCL TR-FRET Assay Buffer*4 ml-20°CWhite, nonbinding, low volume, microtiter plate1Room Temp.* Add 30 µl of 0.5M DTT before use

Shipping Conditions

-80°C

Storage Conditions

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.