Mouse CTLA4[Biotinylated]:B7-1 Inhibitor Screening Chemiluminescence Assay Kit

The Mouse CTLA4[Biotinylated]:B7-1 Inhibitor Screening Chemiluminescence Assay Kit  is an ELISA-based assay designed to measure the binding between mouse CTLA4 (cytotoxic T-lymphocyte associated protein 4) and mouse B7-1 (or CD80) protein for screening and profiling applications. This kit comes with enough purified biotinylated mouse CTLA4 (amino acids 36-161) and mouse B7-1 (amino acids 38-246), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. Mouse CTLA4[Biotinylated]:B7-1 Inhibitor Screening Chemiluminescence Assay Kit schematic.A 96-well plate is coated with mouse B7-1 protein. After coating and blocking, biotinylated mouse CTLA4 is added in an optimized assay buffer. Next, unbound biotinylated mouse CTLA4 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of mouse CTLA4 binding to mouse B7-1.Interested in having us perform the screening and profiling of inhibitors or blocking antibodies? Check out ourImmunotherapy Biochemical Screening Services.