TYR Phosphorylation Assay Kit

TYR (tyrosine) phosphorylation is a modification of proteins where a phosphate group is added to the amino acid tyrosine by tyrosine kinases. This modification is crucial to regulate enzyme activity and signaling pathways, such as the Ras-MAPK (mitogen activated protein kinase) signaling pathway, and is needed for proteins involved in cell differentiation, cell cycle, gene regulation and transcription, angiogenesis and many other functions. Tyrosine kinases can be receptor or non-receptor, with RTK (receptor tyrosine kinases) being transmembrane proteins that can bind ligands extracellularly, while NRTK are mostly soluble intracellular proteins. Tyrosine phosphorylation can be reverted by PTPs (tyrosine phosphatases) . Abnormal tyrosine phosphorylation is linked to several diseases, such as cancer and diabetes. An understanding of the TYR phosphorylation state of a protein and how to manipulate it, via the partners involved, is critical for the development of targeted therapies for tyrosine phosphorylation linked diseases.

Catalog #NameAmountStorage52140Z5Primary Antibody 3012.5 µl-80°C52130HSecondary HRP-Labeled Antibody 110 µl-80°C1x Phosphorylation Buffer3 x 1 ml-20°C79956Blocking Buffer50 ml+4°CPhosphorylated JAK2*50 µl-80°CHRP Chemiluminescence Substrate A (translucent bottle) 6 ml+4°CHRP Chemiluminescence Substrate B (brown bottle) 6 ml+4°C96-well strip plate (coated) 1 plateRoomTemp* The initial concentration of enzyme is lot-specific and will be indicated on the tube containing the protein.

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