IDH1 (R132H) Assay Kit

Isocitrate dehydrogenases are enzymes that catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been reported in a multitude of human cancers. While the wild type IDH1, produces α-ketoglutarate (α-KG) through the reduction of NADP+ to NADPH, the IDH1 (R132H) mutant catalyzes conversion of α-ketoglutarate to 2-hydroxy-glutarate (2-HG) by oxidizing NADPH to NADP+. Genomic studies have identified the IDH1 (R132H) mutation in various cancer types, including glioma, chondrosarcoma, and AML as well as a small percentage of prostate, lung and colon cancers.

CatalogNumberReagentAmountStorage71099IDH1 (R132H) 5 µg-80°CAvoid multiplefreeze/thawcycles!1x IDH1 Assay Buffer20 ml-20°Cα-Ketoglutarate (40 mM) 1 ml-20°CNADPH (500 μM) 2 x 100 μl-20°CNADPH Detection Reagent A2 x 1 mg-20°CNADPH Detection Reagent B2 x 1 mg-20°C7968596-well plate, black2Room Temp

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.