NSD2 Chemiluminescent Assay Kit

The NSD2 Chemiluminescent Assay Kit is designed to measure NSD2 activity for screening and profiling applications. The key to the NSD2 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Product Specifications

UniProt

O96028

Format

Catalog #ComponentAmountStorage51026NSD2 human enzyme*50 µg-80°CAvoidfreeze/thawcycles!52120100 µM S-adenosylmethionine2 x 250 µl-80°C52140P2Primary antibody 16-22 x 12.5 µl-80°C52131HSecondary HRP-labeled antibody 22 x 10 µl-80°C52193Z4x HMT assay buffer 72 x 3 ml-20°C52100Blocking buffer 42 x 50 ml+4°CHRP chemiluminescent substrate A (translucent bottle) 2 x 6 ml+4°CHRP chemiluminescent substrate B (brown bottle) 2 x 6 ml+4°CMicroplate pre-coated with substrate1+4°C*The concentration of NSD2 is lot-specific and will be indicated on the tube containing the enzyme

Shipping Conditions

-80°C

Storage Conditions

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.