FTO Chemiluminescent Assay Kit

The FTO Chemiluminescent Assay Kit is designed to measure FTO activity for screening and profiling applications. The key to the FTO Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to the activity. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, FTO enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against FTO? Check out ourDemethylase Screening ServicesorDNA Replication and Repair Screening Services.This product has beencited 8 times.

Product Specifications

Background

The methylation of internal adenosines at the N6position (m6A) in RNA has been shown to play essential roles in various normal and pathologic processes, including various cancers, leukemia, Alzheimer’s disease, and obesity. FTO (Fat mass and obesity-associated protein), also known as ALKBH9 and BMIQ14, is the first m6A demethylase identified.

UniProt

Q9C0B1

Format

Catalog #ComponentAmountStorage79306FTO100 µg-80°CAvoid freeze/thaw cycles!52140Z4Primary antibody 29100 µl-80°C52131HSecondary HRP-labeled antibody 210 µl-80°C4x FTO assay buffer3 x 1 ml-80°C79556Blocking buffer 150 ml+4°C79670ELISA ECL substrate A (transparent bottle) 6 mlRTELISA ECL substrate B (brown bottle) 6 mlRTWhite 8-well strip plate moduleprecoated with RNA substrate*1+4°C*Make sure you are working in RNAse-free conditions

Shipping Conditions

-80°C

Storage Conditions

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.