CD40:CD40L[Biotinylated] Inhibitor Screening Assay Kit

The CD40:CD40L[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD40:CD40L signaling. This kit comes in a convenient 96-well format, with biotin-labeled CD40, purified CD40L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled CD40 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD40L is coated on a 96-well plate. Next, CD40 is incubated with CD40L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Product Specifications

UniProt

P29965

Applications

This kit is useful for screening for inhibitors of CD40L binding to CD40.

Format

Catalog#ComponentAmountStorage79102CD40, Biotin-labeled50 µg-80 °CAvoidmultiplefreeze/ thawcycles71191CD40L-His10 µg-80 °C793113x Immuno Buffer 150 ml-20 °C79728Blocking Buffer 250 ml+4 °C79742Streptavidin-HRP10 µl-20 °C79670ELISA ECL substrate A (transparent bottle) 6 mlRoom Temp.ELISA ECL substrate B (brown bottle) 6 mlRoom Temp.7969996-well white microplate1+4 °C*The concentrations of CD40 and CD40L are lot-specific and will be indicated on the tubes containing the protein.

Shipping Conditions

-80°C

Storage Conditions

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.