ITK Kinase Assay Kit

The ITK Kinase Assay Kit is designed to measure ITK kinase activity for screening and profiling applications using ADP-Glo®as a detection reagent.

Product Specifications

Background

Tyrosine-protein kinase ITK/TSK, also known as Lyk or IL2-inducible T cell kinase, is a cytosolic tyrosine kinase expressed in T cells. ITK contains protein binding domains SH2 and SH3, which allows the kinase to be recruited by tyrosine-phosphorylated proteins, notably upon activation of the T Cell Receptor (TCR) . It plays an important in TCR signal transduction pathways and ultimately leads to the activation of transcription factor NFAT (nuclear activator of activated T cells), cytokine secretion, and T cell differentiation and expansion.

UniProt

Q08881

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Format

Catalog #NameAmountStorage40445ITK*5 µg-80°C78426Kinase assay buffer 2 (5x) 1.5 ml-20°C79686ATP (500 µM) 100 µl-20°C40217Poly- (Glu4:Tyr) (10 mg/ml) 100 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]