ITK Kinase Assay Kit
The ITK Kinase Assay Kit is designed to measure ITK kinase activity for screening and profiling applications using ADP-Glo®as a detection reagent.
Product Specifications
Background
Tyrosine-protein kinase ITK/TSK, also known as Lyk or IL2-inducible T cell kinase, is a cytosolic tyrosine kinase expressed in T cells. ITK contains protein binding domains SH2 and SH3, which allows the kinase to be recruited by tyrosine-phosphorylated proteins, notably upon activation of the T Cell Receptor (TCR) . It plays an important in TCR signal transduction pathways and ultimately leads to the activation of transcription factor NFAT (nuclear activator of activated T cells), cytokine secretion, and T cell differentiation and expansion.
UniProt
Q08881
Applications
Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Format
Catalog #NameAmountStorage40445ITK*5 µg-80°C78426Kinase assay buffer 2 (5x) 1.5 ml-20°C79686ATP (500 µM) 100 µl-20°C40217Poly- (Glu4:Tyr) (10 mg/ml) 100 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to6 months from date of receipt when the materials are stored as directed.
Notes
Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]
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