CHK1 Kinase Assay Kit
The CHK1 Kinase Assay Kit is designed to measure CHK1 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
Product Specifications
Background
CHK1 is an evolutionarily conserved serine/threonine protein kinase that operates as a cell cycle checkpoint at the G2/M phase transition in response to DNA damage. Activated CHK1 triggers a pleiotropic cellular response via phosphorylation of downstream effectors. For example, Chk1 activates and phosphorylates Wee1 kinase and regulates Cdc25C in response to DNA damage in G2 phase. Its activity is highly controlled at the G2/M phase transition.
UniProt
O14757
Applications
Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Format
Catalog #NameAmountStorage40039CHK1*5 µg-80°C793345x Kinase assay buffer1.5 ml-20°C79686ATP (500 µM) 100 µl-20°C79419CHKtide (1 mg/ml) 500 µl-20°C7969696-well plate, white1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to6 months from date of receipt when the materials are stored as directed.
Notes
Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]
Contraindications
The final concentration of DMSO in the assay should not exceed 1%.
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