ROCK2 Kinase Assay Kit

The ROCK2 Kinase Assay Kit is designed to measure ROCK2 kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Product Specifications

Background

ROCK2, an isoform of ROCK1, is a serine/threonine kinase target of small GTPase Rho that regulates the formation of actin stress fibers and focal adhesions, cytokinesis, and smooth muscle contraction. It is therefore important to proper vascular function and is involved in a number of fibrotic pathologies. The ROCK2 inhibitor Fasudil has been investigated for the treatment of cerebral cavernous malformation (CCM), which causes brain hemorrhages, and is in clinical trials for other applications such as Amyotrophic Lateral Sclerosis (ALS) . Belumosudil, a newer ROCK2 inhibitor, was approved by the FDA for chronic graft versus host disease in 2021.

UniProt

O75116

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Format

Catalog #NameAmountStorage40086ROCK2*1.5 µg-80°C79334Kinase assay buffer 1 (5x) 1.5 ml-20°C79686ATP (500 µM) 100 µl-20°C78387S6K Peptide (1 mg/ml) 500 µl-20°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]