Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2) : ACE2 Inhibitor Screening Colorimetric Assay Kit

The Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2) : ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of the interaction between the SARS-CoV-2 Spike S1 RBD (receptor binding domain) (B.1.1.529 BA.1, Omicron Variant) and human ACE2 (angiotensin-converting enzyme 2) . This kit comes in a convenient 96-well format, with Biotinylated-ACE2 (amino acids 18-740), purified Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant), Streptavidin-HRP, and assay buffers for 100 reactions. The SARS-CoV-2 Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant) protein included in the kit, provides a biologically relevant model for the investigation of SARS-CoV-2/ACE2 interaction.First, SARS-CoV-2 Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant) is coated on a 96-well plate overnight. After washing and blocking, the protein is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP. After a final wash, Colorimetric HRP substrate is added to produce color that can be measured using a UV/Vis spectrophotometer microplate reader. The colorimetric signal is proportional to the efficacy of the binding of Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant) to ACE2.Interested in having us perform the screening and profiling of inhibitors or blocking antibodies? Visit ourCoronavirus Service page.