Spike S1 (B.1.429; Epsilon Variant) (SARS-CoV-2) : ACE2 TR-FRET Assay Kit

The Spike S1 (B.1.429; Epsilon Variant) (SARS-CoV-2) : ACE2 TR-FRET Assay is designed to measure the inhibition of the binding between SARS-CoV-2 Spike S1 (B.1.429; Epsilon Variant) and human ACE2 in a homogeneous 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; the test inhibitor compound is incubated with biotinylated Spike S1, Eu-labeled ACE2, and dye-labeled acceptor for one hour. Then the TR-FRET signal is measured using a fluorescence reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET) .

Product Specifications

Background

The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) . As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein binds to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and human ACE2 may offer some protection against the viral infection.The SARS-CoV-2 variant B.1.429, also known as Epsilon Variant, was first discovered in California, United States. It contains mutations W152C, and L452R and D614G.

Applications

This kit is useful to screen for inhibitors of the interaction between SARS-CoV-2 Spike S1 (B.1.429; Epsilon Variant) and human ACE2.

Format

Catalog #NameAmountStorage100705ACE2, His-Tag, Eu-labeled*2 x 2 µg-80°C101131Spike S1 (B.1.429 Variant), Avi-His-tag, Biotin Labeled (16-685) (SARS-CoV-2) *12.5 µg-80°CDye-labeled acceptor3 x 10 µl-20°C799533x ACE2-Spike TR-FRET Buffer4 ml-20°C79969384-well white microplate1Room Temp*The initial concentration of both ACE2 and Spike S1 is lot-specific and will be indicated on the tube containing the protein.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]

Contraindications

Keep final DMSO concentration at or below 1%.

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