3CL Protease (MERS-CoV) Inhibitor Screening Assay Kit

The 3CL Protease (MERS-CoV) Inhibitor Screening Assay Kit is designed to measure 3CL Protease activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. A protease inhibitor cocktail is included as a positive control.Assay Principle3CL Protease (MERS-CoV) Assay Kit uses a custom-synthesized peptide which has a fluorescence group quenched by a specific quencher. This peptide works as a substrate for 3CL Protease (MERS-CoV) . After proteolytic cleavage, fluorescence product is formed and detected at λexcitation=485 nm and λemission=528 nm.Need us to run inhibitor screens or profile your compounds against 3CL Protease (MERS-CoV) ? Check out ourProtease Screening ServicesorCoronavirus Services.This product has beencited 2 times.

Product Specifications

Background

Middle East Respiratory Syndrome coronavirus (MERS-CoV) poses a significant threat to public health worldwide due to its ability to cause serious human disease with high mortality rates. MERS-CoV has become a global threat due to continuous outbreaks in countries on the Arabian Peninsula and the potential for spread to other countries. MERS-CoV 3CL protease is essential for viral replication; consequently, it is an attractive target for small molecule therapeutics.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Format

Catalog #NameAmountStorage1007573CL Protease, MBP-Tag, His-tag (MERS-CoV) *300 µg-80°C780213CL MERS-CoV substrate (1 mM) 20 µl-80°C78022-M3CL MERS-CoV buffer25 ml-20°CProtease Inhibitor cocktail (10 mM in DMSO) 20 µl-80°C79685Black 96-well plate1Room Temp

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]