Spike Trimer (S1+S2) (B.1.617.2.1; Delta Plus Variant) (SARS-CoV-2) : ACE2 Inhibitor Screening Colorimetric Assay Kit

The Spike Trimer (S1+S2) (B.1.617.2.1; Delta Plus Variant) (SARS-CoV-2) : ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of the interaction between SARS-CoV-2 Spike and human ACE2. The SARS-CoV-2 Spike Trimer, included in the kit, provides a biologically relevant model for the investigation of SARS-CoV-2/host cell interaction.The assay requires only a few steps. First, SARS-CoV-2 Spike Trimer is coated on a 96-well plate overnight. After blocking, the protein is pre-incubated with the inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.

Product Specifications

Background

The COVID-19 pandemic is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) . The Spike glycoprotein is expressed on the surface of the virus as a trimer. Each Spike protein consists of two subunits, S1 and S2, and the S1 subunit contains the receptor binding domain (RBD) which recognizes and attaches to the ACE2 receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells.SARS-CoV-2 Variant B.1.617.2.1, also known as variant Delta Plus, was originally discovered in India and contains deletion Δ156/157 and mutations T19R, G142D, R158G, K417N, L452R, T478K, D614G, P681R, D950N.Drugs targeting the interaction between SARS-CoV-2 Spike protein of and human ACE2 may offer some protection against viral infection. This kit includes theSpike Trimer (S1+S2) (B.1.617.2.1; Delta Plus Variant) (SARS-CoV-2) (BPS Bioscience #101165) in its native trimeric conformation to provide a physiologically relevant screen for inhibitors of the Spike S1:ACE2 interaction.

Applications

This kit is useful for screening inhibitors of ACE2 binding toSpike Trimer (S1+S2) (B.1.617.2.1; Delta Plus Variant) (SARS-CoV-2)

Format

Catalog #NameAmountStorage101165Spike Trimer (S1+S2) (B.1.617.2.1; Delta Plus Variant), His-Tag (SARS-CoV-2) *5 µg-80°C100665ACE2, His-Avi-Tag, Biotin-Labeled *2 x 5 µg-80°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79742Streptavidin-HRP10 µl+4°C79651Colorimetric HRP substrate10 ml+4°C79964Transparent 96-well microplate1Room Temp*The initial concentration of both ACE2 and Spike Trimer is lot-specific and will be indicated on the tube containing the protein.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]