Spike Trimer (S1+S2) (P.1; Gamma Variant) (SARS-CoV-2) : ACE2 Inhibitor Screening Colorimetric Assay Kit

The COVID-19 pandemic is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) . The Spike glycoprotein is expressed on the surface of the virus as a trimer. Each Spike protein consists of two subunits, S1 and S2, and the S1 subunit contains the receptor binding domain (RBD) which recognizes and attaches to the ACE2 receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. The P.1 variant of the SARS-CoV-2 Spike protein (also known as Gamma variant) was originally discovered in Brazil and contains 11 mutations (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I) . Drugs targeting the interaction between SARS-CoV-2 Spike and human ACE2 may offer some protection against viral infection.  This kit includes theSpike Trimer (S1+S2) (P.1; Gamma Variant) (SARS-CoV-2) (BPS Bioscience #100989) in its native trimeric conformation to provide a physiologically relevant screen for inhibitors of the Spike S1:ACE2 interaction.

Catalog #NameAmountStorage100989Spike Trimer (S1+S2) (P.1 Variant), His-Tag (SARS-CoV-2) *5 µg-80°C100665ACE2, His-Avi-Tag, Biotin labeled *2 x 5 µg-80°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79742Streptavidin-HRP5 µl+4°C79651Colorimetric HRP substrate10 ml+4°CTransparent 96-well microplate1Room Temp*The initial concentration of both ACE2 and Spike Trimer is lot-specific and will be indicated on the tube containing the protein.

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