TMPRSS2 Fluorogenic Assay Kit1

TMPRSS2 is a serine protease that facilitates SARS-CoV-2 particle entry into host cells via priming of the viral protein Spike. Its inhibition blocks the fusion of the virus with the plasma membrane after Spike interacts with human receptor Angiotensin-converting enzyme 2 (ACE2), restricting SARS-CoV-2 viral entry, therefore TMPRSS2 is an important therapeutic target for the treatment of COVID-19.The protein may also play a role in prostate cancer because its gene is frequently altered in tumor cells. Indeed, TMPRSS2-ERG and TMPRSS2-ETV1 fusions are frequent, with TMPRSS2-ERG present in approximately 40 to 80% of human prostate tumors. ETS-related gene (ERG) is a transcription factor that regulates the expression of genes involved in embryonic development, cell proliferation and differentiation, and apoptosis, acting as an oncogene. Alternatively, the tumor suppressor function of TMRSS2 is disrupted since the fusion genes arise from deletions that eliminate the TMPRSS2 coding region while juxtaposing its androgen-inducible promoter and the open reading frame of ERG. Overexpression of ERG contributes to development of androgen-independence in prostate cancer through disruption of androgen receptor signaling.

Catalog #NameAmountStorageTMPRSS2*15 µg-80°C78047TMPRSS2 Fluorogenic Substrate (5 mM) 10 µl-20°C (Protect from light) 780481x TMPRSS2 Assay Buffer5 ml-20°C78049Camostat500 µg-80°C7968596-well black plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube

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