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TAK1-TAB1 Kinase Assay Kit

The TAK1-TAB1 Kinase Assay Kit is designed to measure TAK1-TAB1 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in-vitro kinase activity assays.  This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP.  Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.

Product Specifications

Background

TAK1 is a serine/threonine protein kinase that mediates signaling by TGFβ and morphogenetic protein (BMP) . In response to IL-1, TAK1 forms a kinase complex with TAB1 and this complex is required for the activation of nuclear factor kappa B (NF-κB) . TAK1 can also activate MAPK8/JNK and MAP2K4/MKK4 and thus play a role in the cell response to environmental stress. It has been implicated in various inflammation disorders and cancers, including breast and pancreatic cancer.

UniProt

O43318 (TAK1), Q15750 (TAB1)

Format

Catalog #ReagentAmountStorage40279TAK1-TAB1, GST-tag*5 µg-80°CAvoidmultiplefreeze/thawcycles!793345x Kinase assay buffer1.5 ml-20°C79686ATP (500 μM) 100 µl-20°C78514MBP (5 mg/ml) 50 µl-20°C7969696-well plate, white1RoomTemp*The concentration of TAK1-TAB1 is lot-specific and will be indicated on the tube containing the enzyme

Shipping Conditions

-80°C

Storage Conditions

Up to 6 months when stored as recommended.

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