MNK1 Kinase Assay Kit
The MNK1 Kinase Assay Kit is designed to measure MNK1 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in-vitro kinase activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.
Product Specifications
Background
MAPK-interacting serine/threonine-protein kinase 1 (MNK1) is a member of the serine/threonine family that plays a role in mitotic cell cycle progression. Alteration of MNK1 has been shown in various cancers such as breast carcinoma and melanoma. MNK1 phosphorylates eukaryotic translation initiation factor 4E (eIF4E), enhancing phosphorylation of quiescent cells and rendering eIF4E oncogenic.
UniProt
Q9BUB5
Format
CatalogNumberReagentAmountStorage40078MNK140 µg-80°CAvoidmultiplefreeze/thawcycles!793345x Kinase assay buffer1.5 ml-20°C79686ATP (500 μM) 50 µl-20°C40535MBP (5 mg/ml) 100 µl-20°C7969696-well plate, white1RoomTemp
Shipping Conditions
-80°C
Storage Conditions
Up to 6 months when stored as recommended.
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