SARS-CoV-1 Spike Trimer (S1+S2) :ACE2 Inhibitor Screening Colorimetric Assay Kit

The SARS-CoV-1 Spike Trimer (S1+S2) :ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors of the interaction of ACE2 ((angiotensin-converting enzyme 2) with the Spike Trimer (S1+S2) protein. This kit comes in a convenient 96-well format, with purified SARS-CoV-1 Spike Trimer (S1+S2) and ACE2-Biotin (amino acids 18-740) proteins, streptavidin-HRP, colorimetric HRP substrate, and assay buffer for 100 binding reactions. The SARS-CoV-1 Spike Trimer (S1+S2) protein included in the kit, provides a biologically relevant model for the investigation of SARS-CoV-1/ACE2 interaction.Figure 1. Assay Kit schematic.First, SARS-CoV-1 Spike Trimer (S1+S2) is coated on a 96-well plate overnight. After washing and blocking, the protein is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP. After a final wash, Colorimetric HRP substrate is added to produce color that can be measured using a UV/Vis spectrophotometer microplate reader. The colorimetric signal is proportional to the efficacy of the binding of Spike Trimer (S1+S2) to ACE2.Need us to run an inhibitor screening or profiling? Check out ourCoronavirus Biochemical Screening Services.