CD28:B7-2[Biotinylated] Inhibitor Screening Assay Kit

The CD28:B7-2[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD28:B7-2 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-2 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD28 is coated on a 96-well plate. Next, B7-2 is incubated with CD28 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against CD28:B7-2[Biotinylated]? Check out ourImmunotherapy Biochemical Screening Services.

Product Specifications

Background

The activation of naïve T cells requires two signals; the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-2 (CD86) ligand on the APC with the CD28 receptor on the T cell surface. CD28:B7-2 interaction is an important drug target for the regulation of T cells involved in autoimmunity, inflammation, tumor recognition, and immune tolerance.

UniProt

CD28: P10747; B7-2: P42081

Format

CatalogNumberComponentAmountStorage71159B7-2, Biotin-labeled20 µg-80 °CAvoid multiplefreeze/thawcycles71113CD2820 µg-80 °C793113x Immuno Buffer 150 ml-20 °CBlocking Buffer50 ml+4 °CStreptavidin-HRP15 µl-20 °CHRP chemiluminescent substrate A (transparent bottle) 6 ml+4 °CHRP chemiluminescent substrate B (brown bottle) 6 ml+4 °C96-well white microplate1+4 °C

Shipping Conditions

-80°C

Storage Conditions

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.