CD47:SIRP-γ[Biotinylated] Inhibitor Screening Assay Kit

The CD47:SIRP-γ[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD47:SIRP-γ signaling. The key to this kit is the high sensitivity of detection of biotin-labeled SIRP-γ through streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD47 is coated on a 96-well plate. Next, SIRP-γ is incubated with CD47 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against CD47:SIRP-γ[Biotinylated]? Check out ourImmunotherapy Biochemical Screening Services.

Product Specifications

Background

Signal-regulatory protein gamma (SIRP-λ), also known as CD172g, is a cell surface protein expressed mainly by myeloid cells such as macrophages. Its receptor, CD47, is ubiquitously expressed on the surface of all cells and has been found to be overexpressed in some cancers. CD47 binding to SIRP-λ has been shown to be important for T cell trans-endothelial migration (TEM) .

UniProt

CD47: Q08772; SIRP-γ: Q9P1W8

Applications

This kit is useful for screening for inhibitors of SIRP-γ binding to CD47.

Format

Catalog #ComponentAmountStorage71236SIRP-γ, Biotin-labeled5 µg-80°CAvoid freeze/thaw cycles!71177CD4710 µg-80°C79742Streptavidin-HRP15 µl+4°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79670ELISA ECL substrate A (transparent bottle) 6 mlRoom TempELISA ECL substrate B (brown bottle) 6 mlRoom Temp79699White 96-well microplate1+4°C