CD47:SIRP-α[Biotinylated] Inhibitor Screening Assay Kit

The CD47:SIRP-α[Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between CD47 and SIRP-α (Signal-regulatory protein alpha) protein for screening and profiling applications. This kit comes with enough purified CD47 (amino acids 19-139) and biotinylated SIRP-α (amino acids 27-370), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. CD47:SIRP-α[Biotinylated] Inhibitor Screening ChemiluminescenceAssay Kit schematic.A 96-well plate is coated with CD47 protein. After coating and blocking, biotinylated SIRP-α is added in an optimized assay buffer. Next, unbound biotinylated SIRP-α is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of SIRP-α binding to CD47.Need us to run inhibitor screens or profile your compounds against CD47:SIRP-α[Biotinylated]? Check out ourImmunotherapy Biochemical Screening Services.