CD137[Biotinylated]:CD137L Inhibitor Screening Assay Kit

The CD137[Biotinylated]:CD137L Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD137 (also known as 4-1BB) :CD137L (also known as 4-1BB ligand) interaction. This kit comes in a convenient 96-well format, with biotin-labeled CD137 (amino acids 24-186), purified CD137L (amino acids 50-254), streptavidin-labeled HRP, and assay buffer for 100 binding reactions.Figure 1. CD137[Biotinylated]:CD137L Inhibitor ScreeningAssay Kit schematic.A 96-well plate is coated with CD137L protein. After coating and blocking, biotinylated CD137 is added in an optimized assay buffer. Next, unbound biotinylated CD137 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of CD137 binding to CD137L.Need us to run inhibitor screens or profile your compounds against CD137[Biotinylated]:CD137L? Check out ourImmunotherapy Biochemical Screening Services.

Product Specifications

Background

CD137, also known as 4-1BB, is a prototype co-stimulatory molecule expressed on T cells. CD137 interaction with its cognate ligand, CD137L, also known as 4-1BBL, results in increased s T-cell activation and proliferation. Accumulating evidence shows a role for CD137:CD137L signaling in inflammation, suggesting that inhibition of this pathway may provide a therapeutic avenue to treat autoimmune and inflammatory diseases.

UniProt

CD137: Q07011; CD137L: P41273

Applications

Screening for inhibitors of CD137 binding to CD137L.

Format

Catalog#ComponentAmountStorage71171CD137 (4-1BB), His-tagged, Biotin-labeled*2 µg-80 °C71189CD137L (4-1BB ligand), His tagged*10 µg-80 °C79742Streptavidin-HRP10 µl+4°C793113x Immuno Buffer 150 ml-20 °C79728Blocking Buffer 250 ml+4 °C79670ELISA ECL substrate A (transparent bottle) 6 mlRoom Temp.ELISA ECL substrate B (brown bottle) 6 mlRoom Temp.7969996-well white microplate1Room Temp.*The concentration of the protein is lot-specific and will be indicated on the tube.