CTLA4[Biotinylated]:B7-2 Inhibitor Screening Assay Kit
TheCTLA4[Biotinylated]:B7-2 Inhibitor Screening Assay Kitis designed for screening and profiling inhibitors ofCTLA4:B7-2interaction. The key to this kit is the high sensitivity of detection of biotin-labeledCTLA4by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, B7-2 is coated on a 96-well plate. Next, CTLA4-biotin is incubated with B7-2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
Product Specifications
Background
B7-2 (CD86) signaling through CTLA4 (CD152) has been shown to inhibit T cell activation. This co-inhibitory pathway can be overactive in many tumors, enabling cancers to escape the host’s immune system. CTLA4-blocking antibodies, including Ipilimumab (Yervoy) and Tremelimumab, have shown clinical efficacy in treating cancer.
UniProt
CTLA4: P16410; B7-2: P42081
Applications
This kit is useful for screening for inhibitors of CTLA4 binding to B7-2.
Format
CatalogNumberComponentAmountStorage71152CTLA4 (CD152), Fc fusion, Biotin-labeled5 µg-80°C (Avoidfreeze/thawcycles!) 71150B7-2 (CD86), Fc fusion10 µg-80°C79742Streptavidin-HRP15 µl+4°C793113x Immuno Buffer 150 ml-20°CBlocking Buffer50 ml+4°CHRP chemiluminescent substrate A (transparent bottle) 6 ml+4°CHRP chemiluminescent substrate B (brown bottle) 6 ml+4°C79699White 96-well microplate1+4°C
Shipping Conditions
-80°C (Dry Ice)
Storage Conditions
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival
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