PD-1:PD-L2[Biotinylated] Inhibitor Screening Colorimetric Assay Kit

The PD1: PD-L2[Biotinylated] Inhibitor Screening Colorimetric Assay Kit is a colorimetric assay designed for screening and profiling molecules that block the binding of PD-1 (programmed cell death protein 1) and PD-L2 (programmed cell death 1 ligand 2) . This assay kit comes in a convenient 96-well format, with enough purified PD-1 (amino acids 25-167), biotin-labeled PD-L2 (amino acids 20-219), assay buffers and detection reagents for 100 enzyme reactions.The key to this kit is the high affinity of biotin-labeled PD-L2 for streptavidin-HRP. First, a 96-well plate is coated with PD-1, followed by incubation with PD-L2. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader. The signal generated is proportional to the binding between PD-1 and PD-L2.

Product Specifications

Background

PD-L1 and PD-L2 binding to PD-1, a receptor expressed on T-cells, negatively regulates immune responses. PD-1 ligands PD-L1 and PD-L2 are found on the surface of many cancer cells, and their interaction with receptor PD-1 inhibits T cell activity and allows cancer cells to escape immune surveillance. This pathway is also involved in regulating autoimmune responses. Therefore, these proteins (termed immune checkpoints) are promising therapeutic targets for many types of cancer as well as multiple sclerosis, arthritis, lupus, and type I diabetes. Checkpoint inhibitors have remarkable efficacy in a wide range of cancer types and have revolutionized cancer treatment. PD-1 inhibitors nivolumab, pembrolizumab, cemiplimab and PD-L1 inhibitors atezolizumab, avelumab, and durvalumab are all FDA-approved drugs for immuno-therapy.

UniProt

PD-1: Q15116; PD-L2: Q9BQ51

Applications

Screening small molecule inhibitors that block PD-1 binding to PD-L2 for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage71106PD-1 (CD279), Fc fusion (Human) *10 µg-80°C71108PD-L2 (CD273), Fc fusion, Biotin-labeled (Human) *5 µg-80°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79742Streptavidin-HRP10 µl+4°C79651Colorimetric HRP Substrate10 µl+4°C7996496-well transparent plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]