PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit

The PD-L1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to thisPD-1:PD-L1 Inhibitor Screening Assay Kitis the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against PD-1[Biotinylated]: PD-L1? Check out ourImmunotherapy Biochemical Screening Services.This product has beencited 9 times.

Product Specifications

Background

Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses.

UniProt

PD-1: Q15116; PD-L1: Q9NZQ7

Applications

This kit is most useful for screening for inhibitors of PD-L1.

Format

Catalog #ComponentAmountStorage71109PD-1, Biotin-labeled*2 µg-80°CAvoidfreeze/thawcycles!71104PD-L1*10 µg-80°C79742Streptavidin-HRP10 µl+4°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79670ELISA ECL substrate A (transparent bottle) 6 mlRoomTemp.ELISA ECL substrate B (brown bottle) 6 mlRoomTemp.79699White 96-well microplate1+4°C*The concentrations of PD-1 and PD-L1 are lot-specific and will be indicated on the tubes containing theprotein.

Shipping Conditions

-80°C

Storage Conditions

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.