SUV4-20H1 Chemiluminescent Assay Kit

The SUV-20H1 Chemiluminescent Assay Kit is designed to measure the activity of SUV4-20H1 activity for screening and profiling applications. The SUV-20H1 assay kit comes in a convenient 96-well format, with enough recombinant purified SUV4-20H1 (amino acids 2-387), primary and secondary antibodies, S-adenosylmethionine (SAM), pre-coated plate with RNA substrate, blocking buffer and detection reagents for 100 enzyme reactions.Figure 1: Illustration of the SUV-20H1 Chemiluminescent Assay Kit assay principle.

Product Specifications

Background

SUV4-20H1, also known as KMT5B (histone-lysine N-methyltransferase 5B), is a di- and tri-methyltransferase of H4K20. H4K20me2 is one of the most frequent histone modifications, and results from the sequential methylation by KMT5A, followed by SUV4-20H1. Tri-methylation can also occur by SUV4-20H1 and KMT5C. H4K20me3 is found in silenced heterochromatin, found near the centromeres and telomeres where it supports the segregation and integrity of the genome. SUV4-20H1 is structurally similar to SUV4-20H2, and they are mostly redundant. SUV4-20H1 plays a role in development, and abnormalities in this protein can result in genome sensitivity to stress signals and defective DDR (DNA damage repair) . It is thus implicated in cancer, metabolic and neurodevelopmental disorders and aging. SUV4-20H1 is found at higher levels in the prefrontal cortex, and has been linked to autism, possibly by inducing synaptic dysfunction due to alterations in DNA repair and gene regulation. A deeper understanding of the role of this protein and how to manipulate its roles in health and disease may open new therapeutic opportunities in SUV4-20H1 related disorders.

UniProt

Q4FZB7

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

Catalog #NameAmountStorage51090SUV4-20H1, GST-tag*50 µg-80°C8 mM S-Adenosylmethionine500 µl-80°C52140RPrimary Antibody 1825 µl-80°C521604x HMT Assay Buffer 13 ml-20°C52100Blocking Buffer 450 ml+4°C52131HSecondary HRP-Labeled Antibody 210 µl-80°C0.5 M DTT200 µl-20°CHRP Chemiluminescence Substrate A (translucent bottle) 6 ml+4°CHRP Chemiluminescence Substrate B (brown bottle) 6 ml+4°C96-well plate precoated with the histone substrate1+4°C*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Visitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]

Contraindications

The is compatible with up to 1% final DMSO concentration.Use RNAse-free conditions.Avoid strong acids or bases, ionic detergents or high salt conditions.