NSD1 Chemiluminescent Assay Kit

The NSD1 Direct Activity Chemiluminescent Assay Kit is designed to measure NSD1 activity for screening and profiling applications. The key to the NSD1 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against NSD1? Check out ourMethyltransferase Screening Services.

Product Specifications

UniProt

Q96L73

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Format

CatalogNumberComponentAmountStorage51024NSD1 human enzyme100 µg-80°CAvoidfreeze/thawcycles!52120400 μM S-adenosylmethionine500 µl-80°C52140P2Primary antibody 16-212.5 µl-80°C52131HSecondary HRP-labeled antibody 210 µl-80°C52193Z4x HMT assay buffer 73 ml-20°C52100Blocking buffer 450 ml+4°CHRP chemiluminescent substrate A (translucent bottle) 6 ml+4°CHRP chemiluminescent substrate B (brown bottle) 6 ml+4°C96-well plate precoated with histone substrate1+4°C

Shipping Conditions

-80°C

Storage Conditions

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.