NSD2 Chemiluminescent Assay Kit

The NSD2 Direct Activity Chemiluminescent Assay Kit is designed to measure NSD2 activity for screening and profiling applications. The key to the NSD2 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against NSD2? Check out ourMethyltransferase Screening Services.

Product Specifications

UniProt

O96028

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Format

CatalogNumberComponentAmountStorage51026NSD2 human enzyme50 µg-80°CAvoid freeze/thaw cycles!52120100 μM S-adenosylmethionine250 µl-80°C52140P2Primary antibody 16-212.5 µl-80°C52131HSecondary HRP-labeled antibody 210 µl-80°C52139Z4x HMT assay buffer 73 ml-20°C79556Blocking buffer 150 ml+4°CHRP chemiluminescent substrate A (translucent bottle) 6 ml+4°CHRP chemiluminescent substrate B (brown bottle) 6 ml+4°CWhite microplate precoated withhistone substrate1+4°C

Shipping Conditions

-80°C

Storage Conditions

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Notes

Since May of 2018, this kit has been improved with reformulated buffers. The previous version of the NSD2 kit #53009 can still be purchased upon special request.