EHMT1 (GLP) Chemiluminescent Assay Kit

The EHMT1 (GLP) Chemiluminescent Assay Kit is designed to measure EHMT1 (also known as G9a-like protein) activity for screening and profiling applications. The key to the EHMT1 (GLP) Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K9 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by addition of the ELISA ECL substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against EHMT1 (GLP) ? Check out ourMethyltransferase Screening Services.This product has beencited 1 time.

Product Specifications

UniProt

Q9H9B1

Applications

Great for studying enzyme kinetics and HTS applications.

Format

Catalog #ComponentAmountStorage51020EHMT1 (GLP) 4 µg-80°CAvoid freeze/thaw cycles!52140EPrimary antibody 512.5 µl-80°C52130HSecondary HRP-labeled antibody 110 µl-80°C5212020 µM S-adenosylmethionine250 µl-80°C521604x HMT assay buffer 1*3 ml-20°C79556Blocking buffer 150 ml+4°C79670ELISA ECL substrate (2 components) 6 ml eachRoom Temp.96-well plate precoated with histone substrate1 plate+4°C*Add 125 µl of 0.5M DTT.

Shipping Conditions

-80°C

Storage Conditions

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.