PRMT5 TR-FRET Assay Kit

PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. PRMT5 is a critical regulatory-protein linked to genome organization, cell cycle regulation, and stem cell differentiation. PRMT5 transfers methyl groups from S-adenosylmethoinine (SAM) to form mono and symmetric di-methylated arginine residues in histone proteins, transcription factors, and other regulatory proteins.Given its regulatory function, PRMT5 is believed to contribute to tumorigenesis. Its dysregulated activity has been associated with many cancers including lymphomas, lung, and breast cancer.Additionally, PRMT5 has been linked to other diseases including kidney and heart disease as well as neurological disorders such as Huntington’s and Alzheimer’s diseases.

Catalog #ComponentAmountStorage51045PRMT5/MEP50*40 µg-80°CAvoidfreeze/thawcycles!5212060 µM S-adenosylmethionine250 µl-80°CEu-labeled antibody5 µl-80°CBiotinylated histone H4 peptide substrate1000 rxns-80°C4x PRMT5 assay buffer**3 x 1 ml-20°CDye-labeled acceptor2 x 10 µl-20°C78314PRMT5 TR-FRET Detection Buffer4 ml-20°C79969White, nonbinding, low volume, microtiter plate1Room Temp.*The concentration of PRMT5 is lot-specific and will be indicated on the tube containing theenzyme.**Add 10 µl of 0.5 M DTT per vial before use.

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.