PRMT5/MEP50, Biotin-Labeled Recombinant
PRMT5/MEP50 complex of human PRMT5 (GenBank Accession No. NM_006109), amino-acids 2-637 (end), with N-terminal FLAG-Avi tag, MW=74 kDa, and human MEP50 (GenBank Accession No. NM_024102) with N-terminal His-Tag, amino-acids 2-342 (end), MW=38 kDa, coexpressed in HEK293 cells and enzymatically biotin-labeled using Avi-tag™ technology.This product has beencited 2 times.
Product Specifications
Background
UniProt
PRMT5: O14744; MEP50: Q9BQA1
Tag
PRMT5: N-terminal FLAG-Avi-Tag; MEP50: N-terminal His-Tag
Purity
≥ 90%
Format
Aqueous buffer solution
Shipping Conditions
-80°C
Storage Conditions
At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%) .
Calculated Molecular Weight
PRMT5: 74 kDa; MEP50: 38 kDa
Formulation
40 mM Tris-HCl pH 8.0,110 mM NaCl, 2.2 mM KCl, 80 µg/mL FLAG peptide, 20% glycerol and 3 mM DTT.
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