EZH1 Chemiluminescent Assay Kit

The EZH1 Chemiluminescent Assay Kit is designed to measure activity of the EZH1 complex (EZH1/EED/SUZ12/RbAp48/AEBP) for screening and profiling purposes. The key to the EZH1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against EZH1? Check out ourMethyltransferase Screening Services.

Product Specifications

Applications

Useful for studying enzyme kinetics and HTS applications.

Format

CatalogNumberComponentAmountStorage51007EZH1/EED/SUZ12/RbAp48/AEBP225 µg-80°CAvoid freeze/thaw cycles!52120400 μM S-adenosylmethionine250 µl-80°C52140FPrimary antibody 625 µl-80°C52131HSecondary HRP-labeled antibody 210 µl-80°C521704x HMT assay buffer 23 ml-20°C79556Blocking buffer 150 ml+4°C79670ELISA ECL substrate A (translucent bottle) 6 mlRoomTemp.ELISA ECL substrate B (brown bottle) 6 mlRoomTemp96-well plate precoated with histonesubstrate1 plate+4°C