EZH2 (A677G) Chemiluminescent Assay Kit

The EZH2 (A677G) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex [EZH2 (A677G) /EED/SUZ12/RbAp48/AEBP] for screening and profiling purposes. The key to the EZH2 (A677G) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against EZH2 (A677G) ? Check out ourMethyltransferase Screening Services.

Product Specifications

Background

EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.

UniProt

Q15910

Format

COMPONENTS

Shipping Conditions

-80°C

Storage Conditions

6 months from date of receipt when stored as directed.

Notes

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:TBST buffer (1x Tris-buffered saline, pH 8.0, containing 0.05% Tween-20) Luminometer or fluorescent microplate reader capable of reading chemiluminescenceRotating or rocker platformThis mutation is equivalent to A677G in variant 3 (GenBank Accession No. NM_001203247) .