PRMT5 Chemiluminescent Assay Kit

The PRMT5 Chemiluminescent Assay Kit is designed to measure PRMT5 activity for screening and profiling applications. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Product Specifications

Background

PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3.

UniProt

O14744

Applications

Useful for studying enzyme kinetics and HTS applications.

Format

Catalog #ComponentsAmountStorage51045PRMT5/MEP50 human enzyme40 μg-80°CAvoid freeze/ thaw cycles!52120100 μM S-adenosylmethionine250 μl-80°C52150-3Primary antibody 4-3100 μl-80°C52131HSecondary HRP-labeled antibody 22 x 10 μl-80°C4x PRMT5 assay buffer (add DTTbefore experiment) 2 x 3 ml-20°C52100Blocking Buffer 42 x 50 ml+4°CHRP chemiluminescent substrate A (transparent bottle) 2 x 6 ml+4°CHRP chemiluminescent substrate B (brown bottle) 2 x 6 ml+4°C384-well plate precoated with histone substrate1+4°C

Shipping Conditions

-80°C

Storage Conditions

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.