Biotinylated Human CD1d&B2M Monomer Protein (Peptide free, MALS verified)
CD1d is a membrane protein non-covalently bonded to β2-microglobulin (β2m) and shows high homology between human and mice. CD1d can present α-galactosylceramide (α-GalCer), a glycolipid extracted and isolated from the marine sponge, and this complex can activate human and murine CD1d restricted NKT cells.
Product Specifications
Background
CD1d is a membrane protein non-covalently bonded to β2-microglobulin (β2m) and shows high homology between human and mice. CD1d can present α-galactosylceramide (α-GalCer), a glycolipid extracted and isolated from the marine sponge, and this complex can activate human and murine CD1d restricted NKT cells.
Specifications
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 39.0 kDa and 15.7 kDa. The protein migrates as 50-55 kDa and 17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P15813 (CD1d) & P61769 (B2M)
Expression Region
Glu 20 - Ser 301 (CD1d) & Ile 21 - Met 119 (B2M)
Host
HEK293
Target
CD1d & B2M
Conjugation
Biotin-labeled
Tag
C-10xHis & C-Avi
Source
Human
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
0.1 EU per μg
Purity
90%
Bioactivity
Immobilized Biotinylated Human CD1d&B2M Monomer Protein (Cat. No. CDM-H82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human CD1d Antibody, Mouse IgG2b with a linear range of 0.00003-0.125 μg/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
39.0 kDa and 15.7 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
200ug*1
Host or Source
HEK293
Species
Human
Protein ID
P15813 (CD1d) & P61769 (B2M)
Preservative
Trehalose
Curated Selection
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