Anti-MTBP Antibody

Rabbit Polyclonal Antibody specific to MTBP

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

hMTBP

Gene Name

MTBP

NCBI Gene ID

UniProt

Q96DY7

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Peptide corresponding to aa 122-139 of human MTBP. This sequence differs from that of mouse by three amino acids.

Target Antigen

Mdm2-binding protein

Target

MTBP

Clonality

Polyclonal

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Cancer research

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells (By similarity). May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation. {PubMed:15632057}.

Additionnal Information

Immunoblotting: use at 0.5-1 ug/mL. In immunoblots, a band of 104 kD is detected. <br><br>Positive control: K562 cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human and mouse MTBP which is involved in the p53 network of tumor suppression. p53 transcriptionally activates the MDM2 gene, then the translated MDM2 protein binds to p53 and promotes degradation of p53. MTBP binds to MDM2 and inhibits the modulating effect of MDM2 on p53. MTBP is expressed in a variety of normal tissues.