Anti-PUMA (CT) Antibody

Rabbit Polyclonal Antibody specific to PUMA (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

JFY-1, p53 up-regulated modulator of apoptosis

Gene Name

BBC3

Gene ID

27113

NCBI Gene ID

UniProt

Q9BXH1

Accession Number

NP_055232

Cellular Locus

Mitochondrion

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to aa 180-193 of human PUMA-a (accession no NP_055232). This sequence is identical in human PUMA-a and PUMA-b.

Target Antigen

Bcl-2-binding component 3, isoforms 1/2

Target

PUMA (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis (PubMed:11463391, PubMed:23340338). Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis (PubMed:23340338). Regulates ER stress-induced neuronal apoptosis (By similarity). {UniProtKB:Q99ML1, PubMed:11463391, PubMed:23340338}.

Additionnal Information

Immunoblotting: use at 2ug/mL. Positive control: K562 cell lysate. Immunocytochemistry: use at 1ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human and mouse PUMA (23kDa). A lower molecular weight band at 16kDa is detected in MOLT4 and U937 cell lysates and probably represents the PUMA-β protein.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

PUMA (p53 Upregulated Modulator of Apoptosis) is a pro-apoptotic Bcl-2 family member that encodes two BH3 domain- containing proteins, PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to mitochondria, and induce cytochrome c release and apoptosis in response to p53.