Recombinant GZMB Antibody / Granzyme B

Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin s Disease.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P10144

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 73-187 was used as the immunogen for the recombinant GZMB antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RGZMB/6740

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant GZMB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant GZMB antibody is available for research use only.

Storage Conditions

Aliquot the recombinant GZMB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant GZMB antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human tonsil tissue with recombinant GZMB antibody (clone rGZMB/6740) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.