Recombinant Filaggrin Antibody

Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 2-4 µg/mL

UniProt

P20930

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 198-288 was used as the immunogen for the recombinant Filaggrin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

FLG/3909R

Type

Recombinant

Applications

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant Filaggrin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Filaggrin antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Filaggrin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant Filaggrin antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Western blot analysis of human A431 cell lysate using recombinant Filaggrin antibody (clone FLG/3909R) . Expected molecular weight: ~434 kDa (precursor) ; 37-45 kDa (processed) .