Recombinant Aldose reductase Antibody / AKR1B1

AKR1B1, also designated as aldose reductase, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL

UniProt

P15121

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human full-length AKR1B1 protein was used as the immunogen for the recombinant Aldose reductase antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

AKR1B1/7010R

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Aldose reductase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Aldose reductase antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Aldose reductase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Aldose reductase antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human brain tissue with recombinant Aldose reductase antibody (clone AKR1B1/7010R) at 2ug/ml in PBS for 30 min RT. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.